A HeLa cell is shown against a black background with TIS granules shown in purple and the ER in teal.

The cytoplasm contains membraneless compartments, such as the TIS granules (purple) and the ER (teal), where translation occurs. Where an mRNA transcript travels in the cytoplasm determines its expression and whether it forms protein complexes.

credit: Christine Mayr

All about location for mRNA translation

Researchers discovered that where mRNA translation occurs in the cytoplasm changes protein expression level and sometimes even function.
Stephanie DeMarco, PhD Headshot
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Inside every cell is a bustling metropolis. Emerging from the central hub of the nucleus, ribbons of mRNA transcripts spool into the cytoplasmic suburbs ready to be translated into proteins.

“People think cytoplasm is a bag and it doesn't matter where you go because it's all translated there,” said Christine Mayr, a cell biologist at Memorial Sloan Kettering Cancer Center. “What we saw is, first of all, it's not random where the RNA goes, but then it actually matters.”

In a new study, Mayr and her team reported that the cytoplasm is divided into distinct, membraneless compartments, and that where mRNA translation takes place determines both the amount and the function of the resulting protein (1). The discovery may inform the development of new RNA therapies in the future.

Translation occurs on ribosomes in the cytosol or on the endoplasmic reticulum (ER), but in 2018, Mayr and her colleagues discovered that translation of certain mRNA molecules also occurs in a membraneless structure in the cytoplasm called TIS granules (2). TIS granules form a mesh-like structure that wraps around, but remains distinct from, the ER, and they help the proteins translated within them form complexes.

To determine the identities of the transcripts translated in TIS granules as well as on the ER and the cytosol, Mayr and her team isolated the three different structures and performed RNA sequencing on their contents. Much to their surprise, the transcripts enriched at each site had distinct characteristics.

“First, of course, I thought my student did something wrong,” Mayr laughed. “But she repeated it 100 times or something like that, and we always got the same thing.”

People think cytoplasm is a bag and it doesn't matter where you go because it's all translated there. What we saw is, first of all, it's not random where the RNA goes, but then it actually matters. 
- Christine Mayr, Memorial Sloan Kettering Cancer Center

Transcripts encoding small proteins traveled to the cytosol; low abundance protein transcripts and those encoding transcription factors went to TIS granules; and large and highly expressed ones headed to the ER.

Mayr and her team hypothesized that RNA binding proteins shuttled the mRNA transcripts to different compartments, but when they deleted RNA binding proteins and watched where the transcripts went, they realized that mRNA length, exon length, and number of exons in a particular transcript formed a combinatorial code with different RNA binding proteins that determined where a transcript ended up.

“All these architecture features, they actually really play a primary role in this,” she said. “I find that quite beautiful.”

Most surprising, Mayr added, was that redirecting an mRNA to a different location dramatically changed how much of the protein was made and the kinds of protein complexes it formed. When they sent a transcript normally translated in the cytosol to the ER instead, it had a 3.5-fold increase in its protein expression. In a parallel project, the researchers redirected the translation of the cancer oncogene MYC away from its normal location in the TIS granules to the cytosol (3). When translated in the TIS granules, MYC forms protein complexes that regulate MYC target gene expression in the nucleus, but when translated in the cytosol, these complexes do not form, thereby altering MYC’s protein function.

The ability to change a protein’s expression level and function based on where in the cell its translation occurs has exciting implications for RNA and protein drug development.

“My hope is really that in the future, you can make much smarter proteins,” said Mayr. “If it's translated in the cytosol, [it] has function number one. If it's translated in the granule, then it forms a complex, has function number two. You could basically design it like that.”

References

  1. Horste, E.L. et al. Subcytoplasmic location of translation controls protein output. Mol Cell  83, 4509-4523.e11 (2023).
  2. Ma, W. and Mayr, C. A Membraneless Organelle Associated with the Endoplasmic Reticulum Enables 3′UTR-Mediated Protein-Protein Interactions. Cell  175, 1492-1506.E19 (2018).
  3. Luo, Y. et al. mRNA interactions with disordered regions control protein activity. Preprint at: https://www.biorxiv.org/content/10.1101/2023.02.18.529068v1

About the Author

  • Stephanie DeMarco, PhD Headshot

    Stephanie joined Drug Discovery News as an Assistant Editor in 2021. She earned her PhD from the University of California Los Angeles in 2019 and has written for Discover Magazine, Quanta Magazine, and the Los Angeles Times. As an assistant editor at DDN, she writes about how microbes influence health to how art can change the brain. When not writing, Stephanie enjoys tap dancing and perfecting her pasta carbonara recipe.

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