3D tumor models provide a more physiologically relevant model for studying immune-mediated cell killing. Multi-spheroid formats offer additional benefits, including greater throughput and reproducibility, while incorporating an extracellular matrix introduces relevant cell-matrix interactions. However, conventional assays can struggle to image and quantify these complex models and often provide limited temporal and morphological information, making it difficult to capture dynamic immune–tumor interactions.
This application note demonstrates a confocal live-cell imaging workflow for studying immune cell killing in 3D tumor multi-spheroid models, supporting detailed investigation of cell-cell interactions and immunotherapeutic mechanisms of action.
Download this application note to learn how:
- Confocal live-cell imaging addresses challenges in visualizing and analyzing immune cell killing in 3D tumor models
- Immune cells can be visualized at the spheroid interface and within its outer layers
- NK-mediated cytotoxicity and CAR-T cell activity can be evaluated in ECM-embedded spheroids
