Understanding how proteins function and how biotherapeutics perform requires detailed analysis of sequence, structure, and post-translational modifications (PTMs). Recent advances in liquid chromatography–mass spectrometry (LC-MS) have expanded the ability to investigate complex biomolecules with greater resolution, sensitivity, and analytical depth.
This ebook explores how electron capture dissociation (ECD), multidimensional separation approaches, and high-resolution mass spectrometry are advancing protein characterization. Through examples involving proteins, monoclonal antibodies (mAbs), and glucagon-like peptide-1 (GLP-1) analogs, it highlights strategies for sequence analysis, variant identification, and modification mapping in complex biological samples.
Download this ebook to learn:
- How ECD preserves labile PTMs while enhancing protein and peptide analysis
- How multidimensional workflows uncover charge-based heterogeneity in mAbs
- How LC-MS enables comprehensive characterization of GLP-1 analogs
