Ebooks
The future of 3D cell analysis: from isolation to insight
High-throughput, automated approaches are improving how researchers handle, monitor, and analyze complex 3D cell models.
Explore a streamlined approach to protein expression and purification that dramatically reduces timelines and experimental bottlenecks.
Membrane proteins are ubiquitous cellular components that are responsible for a diverse range of cellular and molecular activities, including drug metabolism, antibiotic resistance, and lipid shuttling. Producing membrane proteins for research purposes is plagued with challenges related to detergents that can damage protein structure and function.
Download this application note to learn about:
- An innovative cell-free protein synthesis system that eliminate obstacles to rapid, reliable, and efficient membrane protein production
- Achieving high yield, purity, and reproducibility for drug-relevant membrane proteins
- Scaling up the expression of drug-relevant targets
Top Image Credit:
iStock.com/ spawns
Introduction
Three-dimensional (3D) cell models, such as organoids and spheroids, are transforming medicine by providing crucial
insights for understanding diseases and developing targeted treatments. However, working with 3D cell models presents
unique challenges. These structures are more complex than 2D cultures and require careful handling to maintain their
integrity and viability. They also need frequent monitoring as they grow and mature to ensure the expected function.
Additionally, high-throughput processing is critical for efficiently generating and analyzing large volumes of data, as
traditional methods may not suffice for the scale required in modern research.
Life science instrument technology companies are meeting these demands with high-throughput solutions that support
different stages of the workflow. For monitoring cell health, morphology, maturation, and function, live-cell imaging and
analysis platforms offer unique benefits tailored to 3D culture. First, they provide non-disturbing continuous monitoring
while the cells grow, helping to preserve cellular biology. Second, real-time kinetic tracking offers valuable insights into gene
expression and cell behavior in response to varied conditions. The ability to isolate single cells, spheroids, and organoids with
minimal damage is also crucial for maintaining high viability rates and ensuring the integrity of the research. Automated
systems that can accurately detect, isolate, and transfer 3D structures are invaluable to high-throughput workflows.
This eBook explores the challenges and solutions in working with complex 3D models, emphasizing the importance of
standardized workflows for high-throughput cell manipulation and analysis. It features application notes on culturing,
maintaining, and characterizing induced pluripotent stem cells (iPSCs), as well as label-free, real-time live cell assays for 3D
organoids embedded in Matrigel®. The featured technologies collectively enhance the reproducibility, efficiency, and
physiological relevance of 3D cell models, accelerating breakthroughs from the bench to the bedside.
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Introduction
Three-dimensional (3D) cell models, such as organoids and spheroids, are transforming medicine by providing crucial
insights for understanding diseases and developing targeted treatments. However, working with 3D cell models presents
unique challenges. These structures are more complex than 2D cultures and require careful handling to maintain their
integrity and viability. They also need frequent monitoring as they grow and mature to ensure the expected function.
Additionally, high-throughput processing is critical for efficiently generating and analyzing large volumes of data, as
traditional methods may not suffice for the scale required in modern research.
Life science instrument technology companies are meeting these demands with high-throughput solutions that support
different stages of the workflow. For monitoring cell health, morphology, maturation, and function, live-cell imaging and
analysis platforms offer unique benefits tailored to 3D culture. First, they provide non-disturbing continuous monitoring
while the cells grow, helping to preserve cellular biology. Second, real-time kinetic tracking offers valuable insights into gene
expression and cell behavior in response to varied conditions. The ability to isolate single cells, spheroids, and organoids with
minimal damage is also crucial for maintaining high viability rates and ensuring the integrity of the research. Automated
systems that can accurately detect, isolate, and transfer 3D structures are invaluable to high-throughput workflows.
This eBook explores the challenges and solutions in working with complex 3D models, emphasizing the importance of
standardized workflows for high-throughput cell manipulation and analysis. It features application notes on culturing,
maintaining, and characterizing induced pluripotent stem cells (iPSCs), as well as label-free, real-time live cell assays for 3D
organoids embedded in Matrigel®. The featured technologies collectively enhance the reproducibility, efficiency, and
physiological relevance of 3D cell models, accelerating breakthroughs from the bench to the bedside.
2
