Retroviral integration is a critical step in the human immunodeficiency virus type 1 (HIV-1) life cycle, enabling viral genetic material to become permanently incorporated into the host genome. This process is mediated by the intasome, a large and structurally complex nucleoprotein assembly whose size, flexibility, and heterogeneity have made it difficult to characterize using conventional analytical methods.
This application note details how mass photometry combined with rapid dilution microfluidics can be used to investigate HIV-1 intasome assembly, characterize heterogeneous assembly states, and assess the influence of integrase concentration on complex formation.
Download this application note to learn:
- Why HIV integration complexes are challenging to study using traditional analytical techniques
- How mass photometry can detect and characterize different forms of protein-DNA assemblies in solution
- How protein concentration influences the formation and stability of HIV integration complexes
