Immune cells are constantly making contact — but not every contact has biological meaning. Separating potentially true cell-cell interactions from coincident events is a persistent challenge in immuno-oncology, making it difficult to capture the cellular conversations that drive immune responses.
This application note examines how imaging-enabled flow cytometry combines fluorescence, spatial, and morphological information to identify, analyze, and sort immunological synapses and other biologically relevant cell-cell interactions.
Download this application note to learn:
- How to distinguish true immunological synapses and coincident cell events
- How spatial and morphological imaging parameters improve the analysis of immune cell interactions
- How imaging-enabled flow cytometry can support downstream studies by identifying and sorting biologically relevant cell-cell complexes
