Radioligand therapies combine highly specific targeting molecules with radioactive isotopes to selectively destroy cancer cells. Their complex structures, including highly polar chelators and ligand-linker conjugates, present unique analytical challenges that can complicate chromatographic separation and mass spectrometry detection.
This application note compares reversed-phase liquid chromatography (RP-LC) and hydrophilic interaction liquid chromatography (HILIC) for the analysis of radioligand therapy components, illustrating how different chromatographic approaches influence retention, separation, and detection of polar analytes.
Download this application note to learn:
- Why highly polar chelators can be difficult to retain using conventional RP-LC methods
- How HILIC and RP-LC compare for separating radioligand therapy components
- Analytical considerations that can improve retention, peak shape, and mass spectrometry performance for metal-sensitive analytes
