Cell and gene therapy research often requires inserting large, multi-kilobase DNA sequences into the genome. These multi-kilobase knock-ins help scientists study gene functions and develop new treatments, but verifying insertion accuracy and identifying unintended changes elsewhere in the genome has been challenging. In this webinar, Monica Sentmanat and Anantha Poluri will present long-read sequencing of captured multi-kilobase targets (LOCK-seq), an advanced whole genome profiling method that precisely confirms multi-kilobase knock-ins and detects genome-wide changes, ensuring reliable research models and enhancing the safety of innovative therapies.
Topics to be covered
- Achieving high on-target coverage in knock-in verification
- Strategies for cost-effective LOCK-seq multiplexing and data analysis
- The benefits of nanopore sequencing for cell and gene therapy research
Thursday, March 20th, 2025 | 12:00 PM - 1:00 PM Eastern Time
This webinar will be available to view live and on demand.
Speaker
Monica Sentmanat, PhD
Research Development Lead
Genome Engineering and Stem Cell Center at the McDonnell Genome Institute
Anantha Poluri, PhD
Associate Director, Regional Marketing
Oxford Nanopore Technologies
