Genetic studies have identified thousands of variants associated with immune diseases, yet most occur in non-coding regions where their functional effects remain difficult to determine. High-throughput functional genomics combines CRISPR-based perturbations with single-cell readouts to investigate how regulatory elements influence gene expression in disease-relevant cell types.
This webinar examines a genome-scale approach for mapping immune disease variants in primary human CD4 T cells, using CRISPR interference and targeted perturbation sequencing (TAP-seq) to connect cis-regulatory elements with target genes and downstream pathways.
Watch this webinar to learn:
- How non-coding variants are linked to regulatory elements and genes
- How TAP-seq enables sensitive targeted transcriptional readouts
- How perturbation screens reveal downstream gene regulatory networks
