Tumor cells can differ substantially in the molecular pathways driving their growth and progression, even among patients with the same type of cancer. Because mRNA levels alone may not fully reflect protein abundance, phosphorylation, or signaling activity, understanding these differences requires a more complete view of molecular activity at single cell resolution.
This application note presents simultaneous single cell mRNA and protein analysis of kidney cancer samples, showing how integrated measurements can reveal patient-specific protein signatures, oncogenic signaling, epithelial-to-mesenchymal transition-associated markers, and immune activity within the tumor microenvironment.
Download this application note to explore:
- Simultaneous single cell mRNA and protein analysis from the same sample
- Broad profiling of cell surface, intracellular, and nuclear proteins involved in cancer-related signaling
- Multiplexing and flexible workflows that support efficient sample processing and help reduce batch effects
