Selecting fluorochromes for multiparameter flow cytometry requires balancing several factors that can affect biological resolution and data interpretation. Differences in fluorochrome brightness and spectral spillover, together with antigen density and expression patterns, can influence how clearly cell populations are resolved within increasingly complex panels.
This guide examines how fluorochromes can be prioritized based on resolution and cross-laser spillover, paired with markers according to antigen characteristics, and provides a 17-color flow cytometry panel example.
Download this guide to learn:
- How fluorochrome resolution and cross-laser spillover can inform dye selection during panel design
- How antigen density and expression profiles can guide the pairing of markers with fluorochromes of different brightness and spillover characteristics
- How these selection and pairing principles can be applied when building a flow cytometry panel
