Access More Sequences with
Ansa Biotechnologies
Modern biological experiments require the ability to
base-pair window of high or low GC content. These
interrogate large and diverse sets of DNA. The collection of
regions were then filtered to 144 sequences representing
naturally occurring DNA sequences is immense, and even
an even distribution of local GC content from 10% to 20%
larger is the set of potential synthetic DNA sequences. But
and 80% to 90% across the set of sequences. Ansa
DNA synthesis vendors struggle to deliver a vast portion of
successfully synthesized 96% of these sequences as gene
possible DNA designs. Their processes impose constraints
fragments. Other DNA synthesis companies reject 25% to
on DNA accessibility that have prevented or delayed
58% of these sequences.
countless experiments.
Promoters and 5’ UTRs
To date, no DNA synthesis vendor has devised a process
Promoters and untranslated regions (UTRs) often contain
that can access all DNA sequences, but Ansa’s enzymatic
features that can complicate manufacturing, such as low
synthesis technology greatly increases the number and
GC regions, repeat regions for protein binding, and
diversity of sequences that can now be produced. One of
inverted repeats that can form secondary structures. A
our core strengths is our ability to contiguously synthesize
random sample of promoters with 5’ UTRs (the 600 bp
highly complex oligos up to 600 nucleotides long with
directly 5’ of the start codon) from the human, maize, and
industry-leading accuracy and with almost no sequence
Arabidopsis genomes were synthesized as fragments by
limitations. Using these oligos as input for our unique,
Ansa with a 90% success rate. Other DNA synthesis
sequence-agnostic assembly method, we are now building
companies reject 20% to 65% of these same sequences.
and shipping Ansa Clonal DNA up to 5 kb.
AAV ITRs
Adeno-associated virus (AAV) inverted terminal repeats
Examples of Accessible Sequences
(ITRs) have a characteristic T-shaped secondary structure
that consists of around 150 base pairs. Clonal gene
synthesis of the ITRs from AAV serotypes 1-13 were
Human Genome
manufactured by Ansa and all but one were synthesized
From a random sample of 500 base-pair regions of the
successfully. Other DNA synthesis companies reject all of
hg18 human genome reference, 96% of these sequences
these sequences at the outset or fail to deliver them.
were given a standard complexity rating for clonal gene
synthesis by Ansa, while other DNA synthesis companies
accepted just 63% to 85% of these same sequences. The
GC Content
higher rate of rejection seen by other vendors is largely
associated with complex regions inherent throughout the
genome. As the length of the sequence increases, so does
Ansa can manufacture sequences containing regions of
the probability of the sequence containing complex
high and low GC content, including within a sequence.
features, making it more likely that other vendors will reject
the sequence.
Accepted local GC %
within any 50 base-pair region
Ansa
483
Clonal DNA
10%-94%
Company 1
423
DNA Fragments
6%-90%
Company 2
315
0
100
200
300
400
500
Homopolymers
Sequences Accepted
Long homopolymers present a unique challenge as length
Extreme GC Content
can be di cult to measure using sequencing. Ansa can
In a separate analysis, the human genome was randomly
synthesize long homopolymers and we employ
sampled to identify 600 base-pair regions containing a 50
homopolymer-specific QC measures to assess them.
