Membrane proteins play essential roles in cellular transport, signaling, and immune recognition, and represent many important therapeutic targets. However, studying these proteins remains challenging because they must be removed from their native membrane environment while maintaining structural integrity. Nanodiscs provide a native-like, detergent-free environment that helps preserve membrane protein stability and function.
This application note highlights the use of mass photometry to characterize nanodisc-embedded membrane protein samples during purification, enabling rapid assessment of sample composition, purity, and protein assembly.
Download this application note to learn:
- How mass photometry distinguishes target protein complexes from contaminants and aggregates
- The benefits of nanodiscs for preserving membrane proteins in native-like environments
- Methods for assessing sample quality and validating purified membrane protein preparations
