Charge variants are critical quality attributes in biotherapeutics, influencing stability, biological activity, safety, and immunogenicity. While imaged capillary isoelectric focusing (icIEF) is widely used to separate charge variants, additional characterization methods are needed to identify the molecular changes underlying these differences.
In this webinar, Arnaud Delobel and Nicki Zhang will discuss how icIEF fractionation workflows can enable the collection of separated isoforms for downstream characterization using liquid chromatography-mass spectrometry (LC-MS) and other analytical techniques, with examples spanning fusion proteins, antibody-drug conjugates (ADCs), and adeno-associated viruses (AAVs).
Topics to be covered:
- Collecting separated isoforms from routine icIEF runs for downstream LC-MS analysis
- Comparing biotherapeutics and biosimilars using multiple characterization method
- Applying charge variant workflows to ADCs and AAVs
- Reviewing case studies in antibody fragment and single-domain antibody–drug conjugate (sdAb-DC) characterization
Wednesday, September 23rd 2026 | 10:00 AM – 11:00 AM Eastern Time
This webinar will be available to view live and on demand.
Speakers
Arnaud Delobel, PhD
Director, R&D and Innovation
Quality Assistance
Nicki (Xiaochan) Zhang
Advanced Field Application Scientist
Bio-Techne
