Chimeric antigen receptor (CAR) T cells with similar phenotypes can differ in antigen recognition, activation, persistence, and therapeutic activity. Growing evidence suggests that these functional differences may be reflected in how CARs are spatially distributed and organized on the cell surface.
This application note illustrates how integrating spatial information with flow cytometry enables more comprehensive characterization of CAR T cells, revealing functional diversity that may not be apparent through fluorescence intensity alone.
Download this application note to learn:
- How CAR spatial distribution can distinguish CAR T cell subsets
- How CAR spatial organization can uncover functional heterogeneity
- How integrating spatial and transcriptomic data can provide deeper insight into CAR T cell function and heterogeneity
