White Papers

RT2 Profilerâ„¢ PCR Arrays Pathway-Focused Gene Expression Profiling with qRT-PCR

Pathway-Focused Gene Expression Profiling with qRT-PCR This paper evaluates the performance of the newest technique for monitoring the expression of a panel of pathway- or disease-specific genes: the RT2 Profiler PCR Array System from SABiosciences. The PCR Array System combines the quantitative performance of SYBRR Green-based real-time PCR with the multiple gene profiling capabilities of a microarray. The PCR Array is a 96- or 384-well plate containing RT2 qPCR Primer Assays for a set of 84 related genes, plus five housekeeping genes, and three controls.

Automated Determination of VMA, HVA, 5-HIAA in Urine Using the GilsonGX-274 and Gilson ASPECTM XL

Analyzing catecholamines and their metabolites in patient urine samples is important for sympathoadrenal system tumor diagnosis. This application note discusses the automation of sample preparation and solid phase extraction (SPE) with the CIinRep Complete Kit (RECIPE Chemicals + Instruments GmbH) using the Gilson ASPECTMXL or GX-274 Liquid Handler. Following SPE, fractions were analyzed via HPLC for analytes VMA, HVA, and 5-HIAA using an electrochemical detection (ECD) method.

Gene Synthesis Handbook: Applications and Technologies for DNA synthesis

36-page technical introduction to designing, constructing, and using synthetic genes. What is gene synthesis? How does it differ from traditional molecular cloning? Why is gene synthesis a revolutionary tool, and how is it performed? Includes: case studies from cancer, structural biology, vaccine development, neuroscience, plant biology, and synthetic biology; oligo design tools and assembly methods; and 54 peer-reviewed references.
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