Quantitative reverse transcription PCR (RT-qCR) involves the detection and quantification of RNA. The process is performed by reverse transcription of the enzyme reverse transcriptase, followed by amplification and detection of the specific targets of this DNA using a technique called quantitative PCR (qPCR) or real-time PCR.
Fragment-based lead discovery is establishing itself as valuable approach for the discovery of novel small molecule leads. It relies on establishing a better understanding of the binding mechanism of a ligand for its binding partner by probing for “hot spots “on the protein target with small molecule fragments (typically <300 Daltons). Its simplicity is in the principle of building a pharmacophore from the ground up. Successful selection requires data on binding affinity as well as extensive SAR information provided by a variety of techniques . . .
In flow cytometry and fluorescence microscopy (including high content screening), a reliable estimation of cell viability is important because it is central to assays for apoptosis and in vitro toxicology. Likewise, it is a measure ... (click for more)