White Papers

Download this application note from PHC Corporation of North America to learn how 3D cell culture more closely models in vivo conditions and captures clinically relevant results. 

Quantitative reverse transcription PCR (RT-qCR) involves the detection and quantification of RNA. The process is performed by reverse transcription of the enzyme reverse transcriptase, followed by amplification and detection of the specific targets of this DNA using a technique called quantitative PCR (qPCR) or real-time PCR.  

Determining Nanoparticle Vaccine Construct Design Stability and Antigenic Efficiency by Improving Nanoparticle Vaccine Design with BLI.

Eppendorf performed fed-batch cultures using BioBLU® 1c and 3c Single-Use Vessels controlled in parallel by the new SciVario® twin bioreactor control system.

Download this whitepaper to discover practical applications of data integrity and audit trail review

Pathway-Focused Gene Expression Profiling with qRT-PCR This paper evaluates the performance of the newest technique for monitoring the expression of a panel of pathway- or disease-specific genes: the RT2 Profiler PCR Array System from SABiosciences. The PCR Array System combines the quantitative performance of SYBRR Green-based real-time PCR with the multiple gene profiling capabilities of a microarray. The PCR Array is a 96- or 384-well plate containing RT2 qPCR Primer Assays for a set of 84 related genes, plus five housekeeping genes, and three controls.

Analyzing catecholamines and their metabolites in patient urine samples is important for sympathoadrenal system tumor diagnosis. This application note discusses the automation of sample preparation and solid phase extraction (SPE) with the CIinRep Complete Kit (RECIPE Chemicals + Instruments GmbH) using the Gilson ASPECTMXL or GX-274 Liquid Handler. Following SPE, fractions were analyzed via HPLC for analytes VMA, HVA, and 5-HIAA using an electrochemical detection (ECD) method.

36-page technical introduction to designing, constructing, and using synthetic genes. What is gene synthesis? How does it differ from traditional molecular cloning? Why is gene synthesis a revolutionary tool, and how is it performed? Includes: case studies from cancer, structural biology, vaccine development, neuroscience, plant biology, and synthetic biology; oligo design tools and assembly methods; and 54 peer-reviewed references.