BETHESDA, Md.—Having established efficient pipelines for determining the three-dimensional shapes of proteins, the Protein Structure Initiative (PSI)—sponsored by the National Institutes of Health's National Institute of General Medical Sciences (NIGMS)—is now working to create new mechanisms for sharing the resources it has developed, such as data and expression clones, with the scientific community.
The PSI, which began in 2000, is a ten-year initiative, with the first five years spent developing methods, technologies, and pipelines to speed the structure production process and reduce its cost, according to Dr. Jeremy M. Berg, NIGMS director. The PSI funded a materials repository in September and next year will create an information hub where researchers can search for and submit structural information.
"The products of [our] efforts have been available to the scientific community, but the new resources should dramatically enhance accessibility," he says.
The mission of the PSI, Berg explains, is to make protein structures available from protein sequences or gene sequences. Traditionally, he says, a lab working on a small number of proteins might be able to determine three or four structures over the course of a year.
"So, from the point of view of drug discovery, if there is a particular target that you are interested in, you are, as the PSI moves forward, more likely to have a model of a protein in the databank that you can use as a model to start thinking about whether this is a good drug target," Berg says. "You may not find your protein specifically, but you can get insights about potential binding sites."
"Because evolution reuses protein shapes over and over, you're not confronted with the need to search out every protein encoded in the human genome to understand the function and structure of human proteins," adds Stephen K. Burley, CSO of SGX Pharmaceuticals in San Diego and principal investigator of the New York Structural Genomics Research Consortium, which is one of the centers involved in the PSI.
A key aspect of the PSI, Berg notes, is that all the information is in the public domain, so structures are deposited within six weeks of when they are determined—typically prior to being published in the literature—and are freely available for any researcher to access.
