Induced pluripotent stem cell (iPSC)-derived neuronal models are becoming increasingly important for studying neurological disorders and evaluating pharmacological responses in vitro. To characterize these models, researchers are using high-density microelectrode array (HD-MEA) technology to monitor neuronal activity in real time at both the single-cell and network levels. Yet as experimental scale grows, maintaining consistent cell culture conditions across dozens of wells introduces significant technical variability that can undermine the quality of downstream data.
This application note describes a semi-automated workflow for high-throughput HD-MEA electrophysiology using iPSC-derived neuronal cultures. It also details representative data demonstrating reproducible neuronal network activity and compound-response measurements across multi-well experiments.
Download this application note to learn:
- How semi-automated liquid handling workflows can support scalable HD-MEA experiments while reducing hands-on variability
- A step-by-step protocol spanning cell plating, media exchange, electrophysiology recording, and compound administration
- How scalable electrophysiology workflows can support functional phenotyping, toxicity studies, and drug discovery applications
