Sartorius sends out 4th edition of Live-Cell Analysis Handbook

Handbook includes new assays for organoid culture QC, ATP metabolism and cell cycle, as well as a new chapter on kinetic immune cell assays

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GÖTTINGEN, Germany—Sartorius Stedim Biotech has announced the release of the company’s 4th edition of its Live-Cell Analysis Handbook. The handbook was first launched in 2017 as a comprehensive reference guide for live-cell analysis technologies, with a focus on the complete suite of Incucyte live-cell assays and reagents. It has been well received by Incucyte users, generating over 2,500 requests of the 3rd edition.

“This new edition truly encompasses the broad range of assays that can be accomplished across multiple therapeutic areas, allowing scientists to push the boundaries of cell-based assays. Of particular interest in this edition are assays using live-cell indicators, such as CytoATP, for direct quantification of dynamic ATP changes in complex co-culture models,” said Lindy O’Clair, head of BioA Applications, Reagents and Consumables at Sartorius. “With our unique assay, combining instrumentation, hardware and reagents, we are able to evaluate nutrient exchange between tumor and stromal cells, which can mediate resistance to metabolically-targeted treatments.”

The live-cell applications covered in the handbook include cell culture quality control assays and kinetic assays for cell health, proliferation and viability; cell migration and invasion; protein dynamics and complex cell models; and neuronal models. The 4th edition of the handbook includes new sections on assays for evaluating Organoid Culture QC, ATP Metabolism, and Cell Cycle. There is also a new chapter on kinetic assays for immune cell health and function.

“The 4th edition includes new sections on assays for evaluating more complex live-cell assays, including a solution for locating and tracking organoids in culture to optimize culture conditions, and novel solutions for direct analysis of ATP and cell cycle state,” notes O’Clair. “Additionally, we added a new chapter on kinetic assays for immune cells, allowing for quantitative and dynamic monitoring of health and function.”

“The handbook was written to provide scientists the tools they need to streamline their research and gain deeper biological insights, across a range of assay models,” she continues. “It not only provides details into the data that can be generated using live-cell analysis but also incorporates detailed and optimized protocols to streamline workflows, ultimately driving novel advances in science.”

Sartorius’ Incucyte Live-Cell Analysis System allows for real-time, uninterrupted monitoring of a range of cell types, from proliferating tumor cells to sensitive primary cells. The handbook is written by Sartorius scientists and product managers, and updated yearly to include the newest offerings.

“Incorporating novel indicators, such as the Incucyte CytoATP Lentivirus, allowed us to address problems that were faced using current technologies. The novel reagent allows for ratiometric readouts, providing researchers with direct ATP measurements that are independent of cell number or reporter expression levels, a major hurdle with other traditional assay,” O’Clair states.  

“Using this biosensor, we are also able to characterize the efficacy, selectivity, and mechanisms of compounds that target cancer metabolism in mono- and complex co-culture models, the latter being of significant importance when evaluating the metabolic exchange of cancer cells with stromal cells. Additionally, this assay can be utilized to characterize specific compound effects on OXPHOS to screen for mitochondrial toxicity liabilities,” concludes O’Clair. “Assays such as the Incucyte ATP Assay is thus uniquely positioned to enable researchers to answer important questions using relevant live-cell models.”

Interested readers can download the free 4th Edition Live-Cell Analysis Handbook here.

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