Sample preparation reagent provides reverse transcription-ready RNA in five minutes

Bio-Rad Laboratories Inc. has launched the iScript RT-qPCRSample Preparation Reagent for rapid isolation of total RNA and enablingreverse transcription and real-time PCR to be performed directly from celllysates. iScript employs selective lysis of the cell membrane while leaving thenuclear membrane intact to achieve DNA-free RNA within five to 10 minutes. Thesingle buffer formulation contains all the components needed to perform celllysis, RNA stabilization and genomic DNA removal for sensitive, quantitativegene expression analysis, and it offers sensitive detection of high-, medium-,and low-copy gene targets directly from cell lysates. It also enables multiplexreal-time detection of up to four targets from as few as 10 cells. Uponexposure of cells to iScript, cell disruption and lysis occurs in approximately30 seconds with the aid of mild vortexing. The reagent also stabilizes RNA andeffectively inhibits intracellular ribonucleases. A two-minute, high-speedcentrifugation yields total RNA that can be collected in the supernatant whilethe nuclei containing genomic DNA form a pellet and can be discarded. RNAisolated with iScript is stable for up to six months when stored at -20°C.