The researchers immobilized peptides onto discrete locations on a gold wafer, much as others have spotted DNA microarrays. They then used a form of surface plasmon resonance (SPR) called SPR imaging to perform parallel analysis of multiple binding events on a single chip.
They tested their chip system with a variety of peptides and immune sera and found the system offered high specificity and good regeneration characteristics. Furthermore, using serial dilutions, they found they could detect very small interactions in complex media, and in a manner at least as sensitive as ELISAs. The researchers are confident that by allowing clinicians to identify specific antibodies early, they can facilitate earlier prognosis of disease and therapy optimization.