Introducing mass photometry
for antibody analytics
Antibody-based products are powerful therapeutic agents, but their discovery, development and production
require careful, repeated bioanalytical characterization. Mass photometry (MP) is a powerful tool for
biomolecular analysis that provides information about the mass distribution of a sample at the single-particle
level, in solution and requiring little time and sample. With speed, resolution and ease of use superior to most
other techniques, MP offers transformative capabilities for antibody characterization.
In this e-book, we present case studies demonstrating the
MP offers key advantages that make it an invaluable tool to derisk
capabilities of MP for antibody aggregation (a critical quality
antibody development and production: Measurements take just a
attribute, CQA) and binding assessment. The data showcase
few minutes and require only nanograms of sample.
the strengths of MP for antibody characterization, and how
it compares to frequently used techniques, like size-exclusion
chromatography (SEC).
High-quality information with low resource investment:
1
MP provides data comparable to gold-standard techniques, but with minimal acquisition time (1 minute) and sample
consumption (15-30 ng). Each measurement costs <5$, and the instrument has a small footprint.
Measurements in solution at functionally relevant concentrations:
2
MP measurements are performed in solution – with no column or surface interactions – at nM concentrations, in line
with the usual physiological and functional concentrations of antibodies. If needed, it is possible to measure samples at
µM concentration by using the MassFluidix™ HC microfluidics add-on.
User-friendly and modality-agnostic:
3
MP instruments are easy to use, and sample preparation is straightforward. A simple dilution is enough to measure
antigens and antibodies of any modality separately or in complex samples – saving processing time and minimizing
optimization work.
3
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