Researchers have long sought more efficient ways to measure cell viability, yet many assays still rely on outdated, multistep protocols that introduce variability and inefficiencies. Assessing cell viability is crucial for evaluating cell health, drug efficacy, and cytotoxicity, typically involving methods that distinguish live cells from dead or dying ones based on membrane integrity, metabolic activity, or enzymatic function. However, traditional methods like trypan blue exclusion or enzyme-based assays often require cumbersome handling, lengthy incubations, and lysis steps that can compromise data integrity. These limitations make high throughput screening a challenge, slowing progress in drug discovery, cancer research, and toxicology studies.

With these challenges in mind, researchers at BPS Bioscience developed the LIVE-Step™ Cell Assay System, a luminescence-based solution that simplifies cell viability measurements. This innovative assay provides a homogeneous, single-step workflow that directly quantifies metabolically active cells using ATP-based luminescence. Unlike traditional methods that require multiple reagent additions and washing steps, the LIVE-Ste Cell Assay System simplifies the process by using a single reagent that both lyses cells and measures luciferase activity in one step. Its stable luminescence signal supports high-throughput applications and extended measurement windows, making it ideal for cytotoxicity studies and cell culture optimization. With minimal hands-on time, researchers obtain highly reproducible, quantitative viability data in minutes.

LIVE-Step is transforming research workflows by offering a reliable alternative to conventional assays. Its extended signal stability, lasting up to five hours, allows greater flexibility in experimental design, while its high sensitivity ensures accurate detection at low cell densities. Freeze-thaw stable and compatible with multiple types of media, including those containing phenol red, the assay reduces handling errors and improves consistency across large-scale studies.

As scientific advancements continue to push the boundaries of discovery, tools like LIVE-Step are reshaping how researchers approach cell-based studies. By streamlining workflows and improving data reliability, this assay accelerates the generation of meaningful insights into cell health, proliferation, and treatment effects, driving progress in biomedical research.