Polar and ionizable compounds can present significant challenges in liquid chromatography, including poor retention, limited selectivity, and unwanted interactions with metal surfaces. Selecting an appropriate separation mechanism and stationary-phase chemistry is therefore critical for developing reliable analytical methods across diverse compound classes.
This compendium examines strategies for polar compound analysis using hydrophilic interaction liquid chromatography (HILIC), reversed-phase chromatography, and mixed-mode chromatography, with practical examples spanning radioligand therapy components, pharmaceutical counterions, and highly polar acidic drugs.
Download this compendium to learn:
- How compound polarity and chemical properties influence chromatography column and separation strategy selection
- When HILIC, reversed-phase, or mixed-mode chromatography may be appropriate for challenging polar analytes
- How different approaches can be applied to the analysis of radioligand components, pharmaceutical counterions, and polar drugs
