Cutting the time and cost out of plasmid generation

10-15 BUSINESS DAYS ENFINIA™ PLASMID DNA ENFINIA™ DNA is for research use only. Not for use in diagnostic procedures. ®Elegen Corp. All trademarks are the property of Elegen Corp. unless otherwise specified. All rights reserved. READY-TO-USE SELFAMPLIFYING RNA TEMPLATES Researchers developing RNA therapeutics can save time by ordering clonal, NGS-verified templates for IVT-mediated self-amplifying RNA production. RAPID AI-DRIVEN ANTIBODY OR PROTEIN ENGINEERING In AI-driven antibody or protein engineering, ENFINIA™ Plasmid DNA enables rapid generation of full-length templates for expression, greatly increasing the efficiency of design optimization. ACCELERATE CELL AND GENE THERAPY ENFINIA™ Plasmid DNA supports cell and gene therapy research, including CAR screening, TCR discovery, and lentiviral transfection. Researchers start by ordering and subcloning gene fragments from commercial providers. Depending on the complexity of each fragment, delivery times can extend from days to weeks. Gene fragments are assembled into a vector backbone using cloning methods like restriction digestion, ligation, or Gibson Assembly – all of which require optimization before use. Assembled vectors are transformed into E. coli and plated antibiotic agar before incubating overnight to produce bacterial colonies. DNA from each colony is extracted, purified, and prepped for sequencing. Finally, for long and complex constructs, the DNA from 10 or more prepped colonies must be sequenced to find a colony containing a sequence-perfect plasmid. CUTTING THE COST & TIME OUT Plasmid DNA is a staple in nearly every molecular biology lab, powering critical work from gene cloning and protein production to genome editing and therapeutic development. But for many researchers, building plasmids with long and complex genes is a slow, tedious process fraught with twists and turns. ENFINIA™ Plasmid DNA is the only hassle-free path to clonal plasmid delivered as fast as 15 business days. CONVENTIONAL PLASMID GENERATION Traditionally, each step to building a plasmid takes days of hands-on effort. This comes after waiting weeks or longer for gene fragments to arrive. For long, complex sequences, the efficiency of the multi-fragment assembly process is often poor, requiring multiple attempts. Leveraging Elegen’s patented cell-free DNA cloning technology, ENFINIA Plasmid DNA eliminates fragment subcloning to rapidly produce complex plasmids with gene lengths up to 15 kb. Researchers simply place an order online and receive NGS-verified, sequence-perfect plasmids in as little as 10 business days at an affordable price. PREP — 2-3 DAYS TRANSFORM — 2-3 DAYS SEQUENCE — 1 WEEK OBTAIN — 1-2 WEEKS A SSEMBLE — 2 3- DAYS OF LONG AND COMPLEX CLONAL GENE SYNTHESIS IDENTIFY SEQUENCEPERFECT COLONY EXTRACT AND PURIFY PLASMID DNA TRANSFORM BACTERIA AND GROW COLONIES SUBCLONE GENE FRAGMENTS; INSERT INTO VECTOR BACKBONE OBTAIN GENE FRAGMENTS AND VECTOR BACKBONE

10-15 BUSINESS DAYS ENFINIA™ PLASMID DNA ENFINIA™ DNA is for research use only. Not for use in diagnostic procedures. ®Elegen Corp. All trademarks are the property of Elegen Corp. unless otherwise specified. All rights reserved. READY-TO-USE SELFAMPLIFYING RNA TEMPLATES Researchers developing RNA therapeutics can save time by ordering clonal, NGS-verified templates for IVT-mediated self-amplifying RNA production. RAPID AI-DRIVEN ANTIBODY OR PROTEIN ENGINEERING In AI-driven antibody or protein engineering, ENFINIA™ Plasmid DNA enables rapid generation of full-length templates for expression, greatly increasing the efficiency of design optimization. ACCELERATE CELL AND GENE THERAPY ENFINIA™ Plasmid DNA supports cell and gene therapy research, including CAR screening, TCR discovery, and lentiviral transfection. Researchers start by ordering and subcloning gene fragments from commercial providers. Depending on the complexity of each fragment, delivery times can extend from days to weeks. Gene fragments are assembled into a vector backbone using cloning methods like restriction digestion, ligation, or Gibson Assembly – all of which require optimization before use. Assembled vectors are transformed into E. coli and plated antibiotic agar before incubating overnight to produce bacterial colonies. DNA from each colony is extracted, purified, and prepped for sequencing. Finally, for long and complex constructs, the DNA from 10 or more prepped colonies must be sequenced to find a colony containing a sequence-perfect plasmid. CUTTING THE COST & TIME OUT Plasmid DNA is a staple in nearly every molecular biology lab, powering critical work from gene cloning and protein production to genome editing and therapeutic development. But for many researchers, building plasmids with long and complex genes is a slow, tedious process fraught with twists and turns. ENFINIA™ Plasmid DNA is the only hassle-free path to clonal plasmid delivered as fast as 15 business days. CONVENTIONAL PLASMID GENERATION Traditionally, each step to building a plasmid takes days of hands-on effort. This comes after waiting weeks or longer for gene fragments to arrive. For long, complex sequences, the efficiency of the multi-fragment assembly process is often poor, requiring multiple attempts. Leveraging Elegen’s patented cell-free DNA cloning technology, ENFINIA Plasmid DNA eliminates fragment subcloning to rapidly produce complex plasmids with gene lengths up to 15 kb. Researchers simply place an order online and receive NGS-verified, sequence-perfect plasmids in as little as 10 business days at an affordable price. PREP — 2-3 DAYS TRANSFORM — 2-3 DAYS SEQUENCE — 1 WEEK OBTAIN — 1-2 WEEKS A SSEMBLE — 2 3- DAYS OF LONG AND COMPLEX CLONAL GENE SYNTHESIS IDENTIFY SEQUENCEPERFECT COLONY EXTRACT AND PURIFY PLASMID DNA TRANSFORM BACTERIA AND GROW COLONIES SUBCLONE GENE FRAGMENTS; INSERT INTO VECTOR BACKBONE OBTAIN GENE FRAGMENTS AND VECTOR BACKBONE