Antibody therapeutics have become increasingly sophisticated. Alongside conventional monoclonal antibodies, researchers are now developing bispecific antibodies, antibody-drug conjugates, and other engineered formats designed to improve targeting and efficacy. But as these molecules become more complex, so do the analytical challenges involved in bringing them to the clinic.
Throughout antibody discovery and development, scientists need to answer fundamental questions about their candidates. Has the antibody formed unwanted aggregates? Is it stable under different conditions? Does it bind its target with the expected stoichiometry? These questions are often addressed using multiple techniques, such as size-exclusion chromatography for aggregation analysis and surface plasmon resonance or biolayer interferometry for binding studies, each providing only part of the picture.
Refeyn's TwoMP mass photometer enables rapid, label-free characterization of antibody aggregation, stability, and binding using minimal sample.
Refeyn
Refeyn's TwoMP mass photometer brings several of these measurements together in a single, label-free workflow. Using mass photometry, the benchtop instrument measures individual molecules directly in solution, allowing researchers to rapidly characterize molecular mass distributions while using only nanogram quantities of sample. The technique can distinguish monomers, aggregates, and molecular complexes in just a few minutes without extensive sample preparation.
Beyond assessing sample quality, TwoMP can help researchers study antibody-antigen interactions. By detecting free antibodies, free antigens, and the complexes they form in a single measurement, the system enables analysis of binding stoichiometry and affinity under native solution conditions. This capability is particularly valuable for increasingly complex therapeutic formats, including bispecific antibodies, where multiple binding events can be difficult to resolve using conventional approaches.
As antibody therapeutics continue to evolve, efficient characterization is becoming increasingly important. By enabling rapid assessment of aggregation, stability, and molecular interactions on a single platform, TwoMP helps researchers generate the information needed to advance promising candidates while conserving valuable samples.
