As chimeric antigen receptor (CAR) T cell therapies continue to expand beyond hematological malignancies, researchers are increasingly focused on improving cell quality, functionality, and manufacturing efficiency to support broader clinical applications. Comprehensive characterization has become a critical part of development, providing insights that inform cell quality assessment, manufacturing optimization, and functional evaluation.
This whitepaper examines high-throughput approaches for phenotypic and functional characterization of CAR T cells, highlighting workflows for evaluating activation, memory, exhaustion, cytokine secretion, and tumor cell killing through representative development and assay case studies.
Download this whitepaper to learn:
- Best practices for monitoring CAR T cell phenotype, activation, memory, and exhaustion throughout development and expansion
- Approaches for combining high-throughput cytometry and live-cell analysis to evaluate immune cell function and potency
- Insights from preclinical case studies on assessing CAR T cell performance, specificity, and functional activity
