Potency testing is a critical quality checkpoint before engineered immune cell therapies reach patients. For years, established analytical methods have relied on a single cytokine readout — interferon-gamma secretion. But as regulatory expectations shift toward a more complete picture of immune cell behavior, a narrow functional snapshot is no longer sufficient.
This application note details how a multiplex immunoassay workflow can characterize the broader secretion profiles of chimeric antigen receptor T (CAR T) and natural killer (CAR NK) cells, revealing a level of functional complexity that single-analyte approaches routinely overlook.
Download this application note to learn:
- How to produce functional CAR T and CAR NK cells with high yield and viability
- How screening 123 unique analytes captures immune responses that single-readout assays miss
- How pairing multiplex discovery with automated validation delivers faster, more reproducible results
