| 1 min read
Register for free to listen to this article
Listen with Speechify
0:00
1:00
BALTIMORE—In screening for binding partners for proteins, SPR and bioaffinity chromatography have traditionally been the methods of choice, but the systems can be compromised by debris found in cellular or botanical extracts. Researchers at the National Institutes in Aging and PSS Bio Instruments recently circumvented this fouling problem, however, by adapting bioaffinity techniques to magnetic beads.
 
In a proof-of-concept study published in Analytical Chemistry, the researchers conjugated human serum albumin (HSA) to Bioclone magnetic beads to see if they could reproducibly separate known HSA-binders from known non-binders. They combined the HSA beads with a mixture of six compounds, and then isolated and washed the beads using a Dynal magnetic separator.
 
Using this manual system, the researchers found they could effectively separate the compounds into binding and nonbinding groups. They then repeated the experiment using the automated Magtration System 12GC, and found that the automated system was equally effective. Given these results, the researchers report they will next use the systems to identify protein-protein and protein-ligand interactions from cellular or botanical extracts.

About the Author

Related Topics

Loading Next Article...
Loading Next Article...
Subscribe to Newsletter

Subscribe to our eNewsletters

Stay connected with all of the latest from Drug Discovery News.

Subscribe

Sponsored

GreenCooling

Thermo Scientific™ Centrifuges with GreenCool Technology

Next-generation natural refrigerant cooling system for centrifuges, offering a lower Global Warming Potential and reduced energy consumption.
Clear sample tubes are shown in a clear tote with red lids in a sample prep robot with a blue and silver industrial lab background.

The crucial role of sample preparation in biotherapy manufacturing

Discover how better sample preparation can unlock improved assay accuracy and analytical results.
A black mosquito is shown on pink human skin against a blurred green backdrop.

Discovering deeper insights into malaria research

Malaria continues to drive urgent research worldwide, with new therapies and tools emerging to combat the parasite’s complex lifecycle and global burden.
Drug Discovery News March 2025 Issue
Latest IssueVolume 21 • Issue 1 • March 2025

March 2025

March 2025 Issue

Explore this issue