Residual DNA impurities from plasmid backbones and host cell genomes remain a key safety consideration in recombinant adeno-associated virus (rAAV) manufacturing. Although regulatory frameworks emphasize quantification, conventional polymerase chain reaction (PCR) methods do not distinguish between fragmented DNA and intact sequences with potential biological activity, limiting the depth of risk assessment. This gap has prompted the development of analytical approaches capable of resolving DNA integrity at higher resolution.
In this webinar, David Dobnik and Emily Blair will discuss a first-of-its-kind study using single-molecule linkage analysis to evaluate DNA impurity integrity in rAAV products and its implications in quality control and process characterization.
Topics to be covered:
- Importance of DNA impurity integrity in rAAV safety assessment
- Limitations of conventional PCR in distinguishing DNA fragment types
- Principles of single-molecule linkage analysis for impurity characterization
- Integration of integrity-based measurements into quality control workflows
Thursday, May 21st 2026 | 11:00 AM – 12:00 PM Eastern Time
This webinar will be available to view live and on demand.
Speakers
David Dobnik, PhD
Assistant Professor
National Institute of Biology (NIB)
Emily Blair, PhD
Senior Project Manager
Countable Labs
