Immunotherapies rely on complex interactions between immune cells and tumor cells. As researchers increasingly use three-dimensional tumor spheroid models to better replicate the tumor microenvironment, evaluating immune-mediated tumor cell killing presents new challenges, including limited spatial resolution, assay complexity, and difficulties capturing dynamic cellular behaviors over time. Advanced imaging approaches are helping address these limitations by enabling continuous monitoring of immune cell activity within physiologically relevant models.
This application note explores how confocal live-cell imaging and multiplexed fluorescence readouts can be used to assess immune-mediated killing in three-dimensional tumor spheroids, providing insights into immune cell infiltration, tumor cell death, engineered T cell activity, and antibody-mediated immune responses.
Download this application note to learn:
- Methods for evaluating immune-mediated tumor cell killing in 3D spheroids
- Approaches for monitoring chimeric antigen receptor T cell and natural killer cell activity
- Strategies for assessing immune-tumor interactions over time
