Application Note
Accelerating access to soluble, functional proteins
Discover approaches that shorten the path from DNA constructs to purified, functional proteins.
Traditional protein production methods often involve weeks of trial-and-error to optimize constructs, expression systems, and purification protocols — especially when working with difficult targets. These challenges grow when proteins turn out to be insoluble, unstable, or inactive, making them difficult to use in downstream research. Advances in automated screening and parallelized protein expression now offer a faster route to accessing functional protein variants.
Download this application note to learn:
- How automated expression and purification screens produce soluble, active protein variants in under 48 hours
- Achieving high yield, purity, and reproducibility for drug-relevant proteins
- Confidently scaling up the expression of drug-relevant targets
Top Image Credit:
iStock.com/Love Employee
Shortened timelines to soluble,
active proteins
Conventional methods to optimize and obtain protein can
take weeks to months especially if the expressed proteins
are not soluble, not stable or not active, resulting in a repeat
of the above workflow with new constructs. This leads to
frustration, high cost and time loss.
An automated protein expression and purification system that
performs 192 expression and 30 purification screen in 24 hours.
Scale-up protein expression the next day to get protein in-hand
for downstream applications.
www.nuclera.com
1 Transformation
2
3
5
Cell lysis
6
Verify soluble expression
Protein purification
Weeks 2 days
Order new
Cloning plasmids
7
Analysis of purification
g to mg of proteins
4
Protein expression
Insoluble/low yield. New variants
(up to 50 constructs or more).
Add to Basket
The protein
expression conundrum:
Lengthy project timelines
and laborious workflow
The
solution:
The eProtein DiscoveryTM System
Protein-in-hand in 48 hours
Shortened timelines to soluble,
active proteins
Conventional methods to optimize and obtain protein can
take weeks to months especially if the expressed proteins
are not soluble, not stable or not active, resulting in a repeat
of the above workflow with new constructs. This leads to
frustration, high cost and time loss.
An automated protein expression and purification system that
performs 192 expression and 30 purification screen in 24 hours.
Scale-up protein expression the next day to get protein in-hand
for downstream applications.
www.nuclera.com
1 Transformation
2
3
5
Cell lysis
6
Verify soluble expression
Protein purification
Weeks 2 days
Order new
Cloning plasmids
7
Analysis of purification
g to mg of proteins
4
Protein expression
Insoluble/low yield. New variants
(up to 50 constructs or more).
Add to Basket
The protein
expression conundrum:
Lengthy project timelines
and laborious workflow
The
solution:
The eProtein DiscoveryTM System
Protein-in-hand in 48 hours
