Adeno-associated virus (AAV) vectors are key tools in gene therapy, and maintaining their quality and functionality is crucial. Traditional short-read sequencing methods often struggle with the complex structure of AAV genomes, leading to inaccurate quality control. Long-read Oxford Nanopore sequencing enables the detection of genome truncations, contamination, and accurate characterization of full-length AAV genomes.
Download this application note to learn:
- How to optimize sample preparation for high-quality AAV genome extraction
- Effective library preparation strategies for full-length sequencing of AAV vectors
- Techniques for identifying vector integrity, contamination, and genome modifications
