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Rapid detection and characterization of protein aggregates
Wyatt Technology, the world leader in solutions for absolute macromolecular characterization, has published a new application note to demonstrate the unique capability of its DynaPro Plate Reader dynamic light scattering (DLS) instrument for achieving rapid detection and characterization of protein aggregates. The DynaPro Plate Reader was able to perform DLS measurements on samples within the wells of a well plate, without removing the samples or physically introducing any substances into the wells. As a result, this instrument was able to allow for programmed measurements of many solution conditions with minimum user intervention. The results of the experiment clearly demonstrated that the average size of the samples in solution increase with exposure time, resulting in longer correlation times. The increased hydrodynamic radius and intensity of scattered light were clearly indicative of increased aggregation of proteins with increased exposure to 60°C. Aggregation was not found to evolve significantly over two weeks' storage at 5°C.
Wyatt Technology Inc.