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High-throughput PARP/Apoptosis Assays
April 2008
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Trevigen

Trevigen announces the launch of High-Throughput PARP/Apoptosis Assays for measuring the activity of PARP in 96-well plates. Cell extracts can be obtained using our newly-developed, higher-throughput procedure before, during and after apoptosis induction. During apoptosis, PARP-1 which catalyzes the NAD dependent addition of poly (ADP-ribose) (PAR) onto various cytoplasmic and nuclear proteins, is cleaved from its enzymatically active form (about 116 kDa), to its inactive form (85 kDa). The HT PARP/Apoptosis Assay is an ELISA which semi-quantitatively detects PAR deposited onto immobilized histone proteins (via PARP activity) in a 96-well format. Trevigen offers two formats of the HT PARP/Apoptosis Assay: colorimetric and chemiluminescent. Additional histone-coated strip wells are available separately.

Trevigen
800-873-8443
www.trevigen.com

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